Glutathione-Insulin Transhydrogenase of Human Liver
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چکیده
منابع مشابه
Glutathione-insulin Transhydrogenase of Human Liver.
An enzyme which promotes the degradation of insulin has been isolated from beef liver (2, 3). This enzyme, which has been designated glutathione-insulin transhydrogenase (4), catalyzes the reductive cleavage of the disulfide bonds of insulin by a simple sulfhydryl compound such as glut,athione (4, 5). Because the process of degradation may be important in the etiology of diabetes mellitus, expe...
متن کاملProperties of glutathione insulin transhydrogenase from beef liver.
The glutathione-insulin transhydrogenase from beef liver haa been found to promote the reductive cleavage of the disulfide bonds of insulin by simple sulfhydryl compounds such as glutathione (2). The properties of this enzyme are of interest not only because of its probable involvement in the biological regulation of the level of insulin but also because, at present, it is the only isolated mam...
متن کاملThe effect of hypophysectomy on the glutathione-insulin transhydrogenase activity of rat liver.
Dodge, J., Mitchell, C. & Hanahan, D. (1963) Arch. Biochem. Biophys. 100, 119-130 Fahimi, H. D. & Karnovsky, M. J. (1964) J . Cell Biol. 23, 29A Goldman, R., Kedem, 0. & Katchalsky, E. (1968) Biochemistry 7 , 4518-4531 Goldman, R., Kedem, 0. & Katchalsky, E. (1971) Biochemistry 10, 165-172 Goldstein, L., Levin, Y. & Katchalsky, E. (1964) Biochemistry 3, 1913-1919 Hornby, W. E., Lilly, M. D. & C...
متن کاملStudies on the specificity and mechanism of action of hepatic glutathione-insulin transhydrogenase.
Studies on the specificity and mechanism of action of hepatic glutathione-insulin transhydrogenase, an enzyme capable of catalyzing the reductive cleavage of the disulfide bonds of insulin in the presence of excess reduced glutathione, have been carried out. By coupling reactions catalyzed by glutathione-insulin transhydrogenase to that of glutathione reductase, it was indicated that during the...
متن کاملAssay of Microsomal Membrane-bound Glutathione-insulin Transhydrogenase and Comparison with Protein
1. Inhibition of endogenous microsomal NADPH oxidase by CO enables membranebound glutathione-insulin transhydrogenase (EC 1.8.4.2) to be assayed conveniently by a linked assay involving NADPH and glutathione reductase (EC 1.6.4.2). 2. The specific activity of the enzyme in rat liver microsomal preparations is of the order of 1 nmol of oxidized glutathione formed/min per mg of membrane protein. ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1965
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)97306-0